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Hello

I just measured this and got the following. These were done at room temp
as I didn't have the temp controller hooked up.

E-pure water 1.333
1x HBSS      1.334
1x PBS       1.3345 


Ian Clements
Scientist
Molecular Probes.
Invitrogen Corporation
29851 Willow Creek Road
Eugene, OR 97402
Tel: 541 335 0416
Fax: 541 33 0177
Email:  [log in to unmask]
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Andrew Resnick
Sent: Friday, June 24, 2005 11:06 AM
To: [log in to unmask]
Subject: Q: refractive index of HBSS?

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All,

We are doing some quantitative microscopy on live cells,
including  deconvolution, and we need to know the refractive index of
our
culture medium (green light, 37 degrees C).  Basically, we are using
Hanks'
Balanced  Salt Solution (HBSS) (without phenol red).  I asked
Invitrogen,
but they do not have that information.  If anyone has any idea, please
let
me know- we've been using  n = 1.4 for now.  Thanks in advance,

Andy


Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)