Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

When trying to switch back & forth between FACS & the microscope I find the
Alexa488/Alexa647 pair to be good option. Both are photostable and bright.
They also fit nicely into the FL1 & FL4 windows on most cytometers. In fact,
we usually find it useful to perform our initial 1°/2° titrations on the
FACS. This allows us to quickly scan through varied conditions for minimal
background and high specific fluorescence.

    Hope this helps,

    -Steve


****************************************************************************
Stephen C. Bunnell, Ph.D.
Assistant Professor
Tufts University Medical School
Department of Pathology
Jaharis Bldg., Room 512
150 Harrison Ave.
Boston, MA 02111