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Dear Uriel,

Nunc have a new product that may help, it is called the "LiveCell Array"
and is for Non-adherent Cells. It was developed by Molecular-Cytomics.
It is a coverslip based flow device with tiny indentations etched into
the glass so that cells are held in place, much like eggs in an egg
carton. It is an amazing piece engineering. I would encourage all core
facility managers to have a look at the following web pages. Next time
someone brings you non-adherent cells this could be just what you need.

www.molecular-cytomics.com 
www.nuncbrand.com   then search for "LiveCell Array"

No commercial affiliation with Nunc or Molecular-Cytomics.
Caveat: I have not actually used these devices myself, just seen the
presentations.

Stephen H. Cody

Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute For Cancer Research
PO Box 2008 Royal Melbourne Hospital
Parkville  Victoria    3050
Australia
Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
email: [log in to unmask] 
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----- Original Message -----
From: Uriel TK <[log in to unmask]>
Date: Wednesday, September 13, 2006 2:58 pm
Subject: A cytospin alternative
To: [log in to unmask]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hello,
> 
> We have a lot of trouble accessing a cytospin centrifuge, and that 
> is holding back our capability to do imaging work. We work with 
> immune cells, mainly innate: monocytes, neutrophils, DCs, and some 
> other types of cells here and there. we usually cytospin them and 
> then directly put them in fixative (PFA for fluorescence, ethanol 
> for cytology) before staining them. Is there any way to do without 
> the cytospin step?
> 
> Any comments would be of great help.
> 
> thanks,
> 
> Uriel.
> Uriel Trahtemberg, M.Sc.
> MD/PhD student
> The Laboratory for Cellular and Molecular Immunology
> The Hebrew University - Hadassah Medical Organization
> Jerusalem - ISRAEL 
> 
> "We shall defend our island, whatever the cost may be, we shall 
> fight on the beaches, we shall fight on the landing grounds, we 
> shall fight in the fields and in the streets, we shall fight in 
> the hills; we shall never surrender."
> Winston [Leonard Spencer] Churchill


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