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Dear All

I have the following problem:  We want to quantify abundant approx.1 µm  
bodies in confocal stacks.  The user is oversampling by acquiring 0.1µm  
step size z-stacks through a 100x/1.4 N.A lens on a LSM 510.  However, it  
still appears difficult to be confident  in our interpretation whether a  
given object is continuous or not in neighboring sections due to  
limitations in resolution.  Can we improve our confidence in determining  
the size and number of bodies using deconvolution of these images?  If so,  
what software would be recommended?





-- 
Thomas Stroh, Ph.D.
Director, MNI Microscopy Unit
Montreal Neurological Institute
McGill University
3801 University Street, Room 753a
Montreal, PQ, H3A 2B4, CANADA
Phone: (514) 398-2719, Fax: (514) 398-5871

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