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Clare M. Waterman-Storer, Ph.D.
Associate Professor
Laboratory for Cell Motility Studies
Department of Cell Biology CB163
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla CA, 92037 USA
Office:  858-784-9764
Lab:  858-784-9243
Fax:  858-784-9779
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Robert Zucker
Sent: Monday, November 06, 2006 7:58 AM
To: [log in to unmask]
Subject: Re: Spectral and Hyperspectral imaging-special issue Cytometry part
A V 69 2006

Spectral and Hyperspectral imaging now offers an exciting and dynamic
tool for biological research. Consider, that at the present time all
confocal microscopes offer spectral capabilities.  However, spectral
imaging (essentially mapping spectra) began with wide field applications
using classical spectroscopic techniques such as reflection,
absorption not just fluorescence. Due to the rapid changes in this
field, a special issue of Cytometry Part A has been devoted to this
topic to help set a foundation for current research and future research.

The editors of this special issue, Robert Zucker, Jeremy Lerner and
Steve Lockett hoped that this issue would help to provide a solid
introduction to the biological spectroscopy field; and to stimulate
future research. The issue contains 23 papers consisting of reviews,
research reports and technical notes.  They present a very wide range of
subjects including: Confocal spectral imaging, a tutorial on how imaging
spectrometers work, FRET and Raman mapping, calibration, spectral
unmixing; and the use of absorption for characterizing ion-transport
through membranes. Everyone involved in this aspect of confocal
microscopy, or wanting to get involved in this exciting field, is
encouraged to read this issue.  Please contact the individual authors or
one of the editors Robert Zucker  ([log in to unmask]) or (Jeremy
Lerner [log in to unmask]) or Stephen Lockett (
[log in to unmask]) for further information on any of the articles. We
hope you enjoy the issue.

Editorial (p 711)
   Jeremy Lerner, Steve Lockett, Robert Zucker

Review articles
Jeremy M. Lerner: “Imaging spectrometer fundamentals for researchers in
the biosciences  - A tutorial” (p 712-734)

Yuval Garini, Ian T. Young, George McNamara: “Spectral imaging:
Principles and applications” (p 735-747)

Richard M. Levenson, James R. Mansfield: “Multispectral imaging in
biology and medicine: Slices of life” (p748-758)

Quentin S Hanley, Patricia I Murray, Toni S Forde: “Microspectroscopic
fluorescence analysis with prism-based imaging spectrometers: Review and
current studies” (p 759-766)

Robert C. Leif, Lidia M. Vallarino, Margie C. Becker, Sean Yang:
“Increasing the luminescence of lanthanide complexes” (p 767-778)

Luis G. Rodriguez, Stephen J. Lockett, Gary R. Holtom: “Coherent
anti-stokes Raman scattering microscopy: A biological review” (p
779-791)

Original Articles
Robert E. Gyurcsányi, Ernõ Lindner Multispectral imaging of ion
transport in neutral carrier-based cation-selective membranes” (p
792-804)

Kerenza L. Njoh, Laurence H. Patterson, Mire Zloh, Marie Wiltshire,
Janet Fisher, Sally Chappell, Simon Ameer-Beg, Yanhong Bai, Daniel
Matthews, Rachel J. Errington, Paul J. Smith: “Spectral analysis of the
DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells”
(p 805-814)

Don Clark, Slobodan Sasic: “Chemical images: Technical approaches and
issues” (p 815-824)

Jeffrey M. Larson: “The Nikon C1si combines high spectral resolution,
high sensitivity, and high acquisition speed” (p 825-834)

Paul M. Kasili, Tuan Vo-Dinh: “Hyperspectral imaging system using
acousto-optic tunable filter for flow Cytometry applications” (p
835-841)

Gregory Goddard, John C. Martin, Mark Naivar, Peter M. Goodwin, Steven
W. Graves, Robb Habbersett, John P. Nolan, James H. Jett: “Single
particle high resolution spectral analysis flow cytometry (p 842-851)

William E. Ortyn, Brian E. Hall, Thaddeus C. George, Keith Frost, David
A. Basiji, David J. Perry, Cathleen A. Zimmerman, David Coder,Philip J.
Morrissey: “Sensitivity measurement and compensation in spectral
imaging” (p 852-862)

George McNamara, Amit Gupta, James Reynaert, Thomas D. Coates, Carl
Boswell: “Spectral imaging microscopy web sites and data” (p 863-871)
A. Thomas Harris: “Spectral mapping tools from the earth sciences
applied to spectral microscopy data” (p872-879)

Andrew Whitley, Fran Adar: “Confocal spectral imaging in tissue with
contrast provided by raman vibrational signatures” (p880-887)

Eunah Lee, Linda H. Kidder, Victor F. Kalasinsky, Joseph W. Schoppelrei,
E. Neil Lewis: “Forensic visualization of foreign matter in human tissue
by near-infrared spectral imaging: Methodology and data mining
strategies” (p 888-896)

Lisa L. Nuffer, Patricia A. Medvick, Harlan P. Foote, James C. Solinsky:
“Multispectral/hyperspectral image enhancement for biological cell
analysis” (p 897-903)

Christopher Thaler, Steven S. Vogel: “Quantitative linear unmixing of
CFP and YFP from spectral images acquired with two-photon excitation” (p
904-911)

V. Krishnan Ramanujan, Eva Biener-Ramanujan, Kinton Armmer, Victoria E.
Centonze, Brian A. Herman: “Spectral kinetics ratiometry: A simple
approach for real-time monitoring of fluorophores  distributions in
living cells” (p 912-919)

Kurt I. Anderson, Jeremy Sanderson, Silke Gerwig, Jan Peychl: “A new
configuration of the Zeiss LSM 510 for simultaneous optical separation
of green and red fluorescent protein pairs” (p 920-929)

Technical Notes
Robert M. Zucker, Susan C. Jeffay: “Confocal laser scanning microscopy
of whole mouse ovaries: Excellent morphology, apoptosis detection, and
spectroscopy” (p 930-939)

Robert C. Leif, Lidia M. Vallarino, Margie C. Becker, Sean Yang
Increasing lanthanide luminescence by use of the RETEL effect (p
940-946)
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory
Reproductive Toxicology Division, MD 67
2525 E.NC Highway 54
Research Triangle Park, North Carolina, 27711
Tel: 919-541-1585; fax 919-541-4017
e-mail: [log in to unmask]