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The dean came into our meeting and we just got out.  will you be  
available at 5 pm?
On 30-Nov-06, at 4:11 PM, Gert van Cappellen wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> The the Zeiss LSM510 series can be supplied with a so called  
> monitor diode. This diode gives a relative laser power signal as an  
> extra channel in your image. It can be used to normalise your image  
> pixel by pixel. In our FRAP experiments we saw nice correlations  
> between fluorescent fluctuations and the laser power fluctuations.
>
> Kind regards,
> Gert van Cappellen
> Optical Imaging Centre Erasmus MC, Netherlands
> www.erasmusmc.nl/oic
>
> Peng Xi schreef:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi,
>>     Does anybody know how the laser intensity is monitored in a  
>> commercial confocal system? How the laser fluctuation (they are  
>> very small,+/-3%) affects the fluorescence image collection? How  
>> it is corrected if it exists?
>>     Thank you!
>>
>> Sincerely,
>> Peng Xi
>> Purdue University Cytometry Laboratories
>> Bindley Bioscience Center
>> 1203 W. State Street
>> West Lafayette, IN 47907
>> Tel: 765-494-0757
>> Fax: 765-494-0517
>> http://web.ics.purdue.edu/~pxi
>>