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November 2006

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Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 8 Nov 2006 07:52:47 -0600
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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Stain for : Micro crack penetration, single crystals
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From:
Martin Wessendorf <[log in to unmask]>
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COETZEE, S.H. (MR.) wrote:

> A user want to stain microcracks in single crystals.  The dye will need to be a small molecule with a good wettability, high mobility.
> Sussetions?  

How about something old: fluorescein (rhodamine B should work, too)? 
Both dyes are water soluble and wetability could be increased by adding 
detergent or alcohol.  Penetration could also be helped by first soaking 
the crystal in (low) vacuum in ethanol or methanol and then placing it 
in the dye solution.

Good luck!

Martin Wessendorf
-- 
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu

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