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Sorry, there is indeed a typo - I wrote 'slide' when I meant
to write 'slice' - probably a finger reflex!  I don't work
on brains - if the cells are intact and alive you can of course
measure a membrane potential but presumably some at least of the
neurons must have been cut in making the slice?  Even if you can
measure an action potential electrophysiologically I can't see
how you can get a membrane potential with D18-ANNEPS unless
the cell has an intact and functional (polarized) membrane.

						Guy

Philip Oshel wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> I'm not so sure about that. Action potentials can be gotten from dead 
> neurons. I worked in a lab once doing EM for a bunch of 
> neurophysiologists, and they routinely got action potentials not only 
> from dead brain slices, but also slilces where the neurons had been dead 
> long enough to lose their membranes. (Needless to say, they never liked 
> being told this.)
> 
> But, I think there's a typo here. Tino refers to "slice preparation", 
> which is a tissue sample thick enough to contain living, intact cells, 
> not to "slide preparation".
> 
> Phil
> 
> 
>> Yes, but I don't quite understand what Tino means by
>> slide preparations.  You'll only get a measure of
>> membrane potential is the cells are intact and alive.
>>
>>                     Guy
>>
>> moreno wrote:
>>
>>>  yes,
>>>  DI8-ANNEPS for example.
>>>  Tino Jaeger wrote:
>>>
>>>>  Hi,
>>>>
>>>>  is there any fluorescence dye, which can can be used to code for the
>>>>  membrane potential of cells (e.g. loading of the cells like with 
>>>> FURA-AM,
>>>>  and the then fluorescence intensity of the dye shows up the different
>>>>  voltage (membrane potential) of cells in a slice preparation)?
>>>>
>>>>  Thanks for your help.
>>>>  Tino
>>>>
>>>
>>
>> -- 
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Electron Microscope Unit, Madsen Building F09,
>> University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>> http://www.guycox.net
> 
> 

-- 
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net