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Did they do the control of leaving out the cells entirely? Where did
you publish?
At 04:58 AM 11/9/2006, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Yeah, sounds wierd -- but what what I meant was the cells were dead
>and they were getting action potentials, and the experiments were
>continued long enough for the membranes to be lost also, not that
>the action potentials were coming from dead cells with no membranes.
>I wan't the only one who noticed this.
>Phil
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Hmm action potentials without membranes? I had better revise my
>>teaching on the biophysics of cells!
>>
>>LOL
>>
>>Cheers
>>
>>
>>Philip Oshel wrote:
>>
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> I'm not so sure about that. Action potentials can be gotten from
>>> dead neurons. I worked in a lab once doing EM for a bunch of
>>> neurophysiologists, and they routinely got action potentials not
>>> only from dead brain slices, but also slilces where the neurons
>>> had been dead long enough to lose their membranes. (Needless to
>>> say, they never liked being told this.)
>>>
>>> But, I think there's a typo here. Tino refers to "slice
>>> preparation", which is a tissue sample thick enough to contain
>>> living, intact cells, not to "slide preparation".
>>>
>>> Phil
>>>
>>>
>>>> Yes, but I don't quite understand what Tino means by
>>>> slide preparations. You'll only get a measure of
>>>> membrane potential is the cells are intact and alive.
>>>>
>>>> Guy
>>>>
>>>> moreno wrote:
>>>>
>>>>> yes,
>>>>> DI8-ANNEPS for example.
>>>>> Tino Jaeger wrote:
>>>>>
>>>>>> Hi,
>>>>>>
>>>>>> is there any fluorescence dye, which can can be used to code for the
>>>>>> membrane potential of cells (e.g. loading of the cells like
>>>>>> with FURA-AM,
>>>>>> and the then fluorescence intensity of the dye shows up the different
>>>>>> voltage (membrane potential) of cells in a slice preparation)?
>>>>>>
>>>>>> Thanks for your help.
>>>>>> Tino
>>>>
>>>> -- ______________________________________________
>>>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>>>> Electron Microscope Unit, Madsen Building F09,
>>>> University of Sydney, NSW 2006
>>>> ______________________________________________
>>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>>>> Mobile 0413 281 861
>>>> ______________________________________________
>>>> http://www.guycox.net
>>>
>
>--
>Philip Oshel
>Microscopy Facility Supervisor
>Biology Department
>Central Michigan University
>024C Brooks Hall
>Mt. Pleasant, MI 48859
>voice: (989) 774-3576
>dept. fax: (989) 774-3462
George McNamara, Ph.D.
Glendale, CA
818-547-6909
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