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Hi and thanks for the responses!

Let me explain again my question.

I try to find a tool for the following question:
I wonder if in a pain-model I use a subset of neurons is depolarized.
To patch the cells would influence the resting membrane potential of the 
neurons.
Therefore I got the idea to stain the slice with some fluorescence dye 
and to see (e.g. by the fluorescence intensity) if there is a subset of 
neurons with a more positive resting potential.
(I do not need to record voltage changes upon stimulation at that point).

Are DI8-ANNEPS or DI4-ANEPPDHQ etc applicable to answer my questions?

Thanks a lot for your help!

Tino




Philip Oshel schrieb:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I'm not so sure about that. Action potentials can be gotten from dead 
> neurons. I worked in a lab once doing EM for a bunch of 
> neurophysiologists, and they routinely got action potentials not only 
> from dead brain slices, but also slilces where the neurons had been 
> dead long enough to lose their membranes. (Needless to say, they never 
> liked being told this.)
>
> But, I think there's a typo here. Tino refers to "slice preparation", 
> which is a tissue sample thick enough to contain living, intact cells, 
> not to "slide preparation".
>
> Phil
>
>
>> Yes, but I don't quite understand what Tino means by
>> slide preparations.  You'll only get a measure of
>> membrane potential is the cells are intact and alive.
>>
>>                     Guy
>>
>> moreno wrote:
>>
>>>  yes,
>>>  DI8-ANNEPS for example.
>>>  Tino Jaeger wrote:
>>>>  Hi,
>>>>
>>>>  is there any fluorescence dye, which can can be used to code for the
>>>>  membrane potential of cells (e.g. loading of the cells like with 
>>>> FURA-AM,
>>>>  and the then fluorescence intensity of the dye shows up the different
>>>>  voltage (membrane potential) of cells in a slice preparation)?
>>>>
>>>>  Thanks for your help.
>>>>  Tino
>>>>
>>>
>>
>> -- 
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Electron Microscope Unit, Madsen Building F09,
>> University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>> http://www.guycox.net
>


-- 
Dr. Tino Jäger
Department of Neurophysiology 
Center for Brain Research
Medical University of Vienna 
Spitalgasse 4, A-1090 Vienna

Tel:  +43-1-4277-62847
Secr: +43-1-4277-62835 
FAX:  +43-1-4277-62865

http://www.meduniwien.ac.at