CONFOCALMICROSCOPY Archives

November 2006

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From:
Artem Pliss <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 14 Nov 2006 17:48:11 -0500
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello Sophie,
For me pure ether always worked best. Some people recommend 1:1 mixture of 
ether with ethanol. Also, you may try a regular lighter fluid.
Regards,
Art

--On Tuesday, November 14, 2006 4:22 PM -0600 "S. Brunet" 
<[log in to unmask]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello to all,
>
> I have a user who mixed two immersion oil types (Immersol 518F and
> Cargille 37).  I tried to clean it with hexane (worked fine on the 37 oil
> before when not contaminated with the 518F), acetone, methanol, and
> ethanol using Kodak lens paper (lots of it).  (OK, I did not expect the
> last two solvents to work...)  The bulk is gone, but there is goop left
> on the lens.
>
> What else can I use?  It seems that 'I drag the goop around' when I clean
> the lens.  I do not want to soak the oil immersion objective in an
> organic solvent for longer than necessary.  This objective is heavily
> used and I am trying to avoid buying a new one (and the down time of
> waiting for a new one).  Xylene does not sound good for the glue.
> Toluene and glue... ?  CFCs?  Dawn soap?
>
> Regards,
> Sophie
> _______________________________________
> Sophie M.K. Brunet, Ph.D.
> Research Officer
> Optical Spectroscopist, Laser Systems and Applications
> [log in to unmask]
> 306-966-1719 (office)   306-966-1702 (fax)
> _______________________________________
> Saskatchewan Structural Sciences Centre
> University of Saskatchewan
> Thorvaldson Bldg.
> 110 Science Place
> Saskatoon, SK   S7N 5C9
>
>




Artem Pliss, Ph.D.
Department of Biological Sciences
State University of New York at Buffalo
Cooke Hall #628, Buffalo, NY, 14260
Phone: 716-645-2363 (ext 156/157)
Fax: 716-645-2975

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