Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
OK, here is a radically different way to do it.
First off, make absolutely sure there is no
trace of xylene or other solvent on the lens.
Take a piece of styrofoam, fracture it and press
the freshly exposed (and therefore clean) surface
against the lens and rotate. It works brilliantly.
I was shown the method by a Leica service techo.
It's detailed in my new book Optical Imaging
Techniques in Cell Biology published by CRC
(shameless plug). (Appendix 1, Microscope care
and maintenance) http://www.guycox.com/optical.htm
Guy
S. Brunet wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello to all,
>
> I have a user who mixed two immersion oil types (Immersol 518F and Cargille 37).
> I tried to clean it with hexane (worked fine on the 37 oil before when not
> contaminated with the 518F), acetone, methanol, and ethanol using Kodak lens
> paper (lots of it). (OK, I did not expect the last two solvents to work...)
> The bulk is gone, but there is goop left on the lens.
>
> What else can I use? It seems that 'I drag the goop around' when I clean the
> lens. I do not want to soak the oil immersion objective in an organic solvent
> for longer than necessary. This objective is heavily used and I am trying to
> avoid buying a new one (and the down time of waiting for a new one). Xylene
> does not sound good for the glue. Toluene and glue... ? CFCs? Dawn soap?
>
> Regards,
> Sophie
> _______________________________________
> Sophie M.K. Brunet, Ph.D.
> Research Officer
> Optical Spectroscopist, Laser Systems and Applications
> [log in to unmask]
> 306-966-1719 (office) 306-966-1702 (fax)
> _______________________________________
> Saskatchewan Structural Sciences Centre
> University of Saskatchewan
> Thorvaldson Bldg.
> 110 Science Place
> Saskatoon, SK S7N 5C9
--
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
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