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Gary Laevsky wrote:

> We're taking some multimodal images of skin (SHG, 2P (auto), 
> reflectance, and Raman), 20-60x.
> 
> The SHG, 2P, and reflectance are on one scope and the Raman is on 
> another (pretty cool images in all modes).
> 
> We've reached a quandary on how to register these images (even get near 
> similar fields of view, which are between 50-250 um).
> 
> A pen mark would be way too big.  Too many hair follicles to narrow down 
> to.  Latex beads will probably float away (the skin needs to stay 
> hydrated).  A grid coverslip? Maybe a gene gun?
> 
> The scopes are in different buildings, so stability of the "mark" IS an 
> issue.

How about this: place the specimen on a hard surface (e.g. a glass 
slide).  Poke it with a glass micropipette.  That should leave a small 
hole in the tissue embedded with glass shards.  You'll probably need a 
micromanipulator to do this right but any slice electrophysiologist 
should be able to help you out.

Good luck!

Martin Wessendorf
-- 
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu