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The efficiency of exciting fluorochromes is only
part of it.  473 will be less good than 488 at
exciting FITC but the window for detecting FITC
will be much wider with the combination Maria
proposes, so in the long run she'll probably do better.
That does depend on a custom triple dichroic being
available for the 473, 559 and 635 combination.
Without that there will be a problem ...

                                           Guy



> Hi Maria,
> If you go to either of these sites, you can see where common laser lines
> intersect the the spectra of fluorescent dyes and proteins, and hence
> their
> efficiency.  This, of course, does not take into account the power output
> of
> the lasers.
>
> http://www.mcb.arizona.edu/IPC/spectra_page.htm
>
> http://www.mcb.arizona.edu/ipc/fret/
> Cheers,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709
> ----- Original Message -----
> From: "Maria Jimena Ortega" <[log in to unmask]>
> To: <[log in to unmask]>
> Sent: Monday, March 12, 2007 2:30 PM
> Subject: lasers for LSM
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>
> Hi,
> we use a system with 488, 543 and 633 lasers for common fluorochromes.
> We are thinking of changing to a system with the 473, 559 and 635 diodes
> lines.
> Does anyone have a comparison of the eficiency of this lines for most used
> fluorochromes?
>
> Thanks a lot
>
> Maria
>


-- 
Associate Professor Guy Cox
Electron Microscope Unit,
University of Sydney,
NSW 2006, Australia

Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
http://www.guycox.net