Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I have no commercial interest in Chroma but I'd say my experinece with
Chroma is the opposite to yours. They did a great job making some custom
filters at a very good price a little while ago and were very quick at
getting the initial (design) filter characteristic back to me. Of
course things may have changed...
Cheers Mark
Kevin Braeckmans wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>As a side note, there are also solid state lasers available with a 490nm
>line (Crystalaser), a 491nm line (Cobolt - up to 100mW!) and even 488 nm
>(Point Source).
>
>As for custom filter sets, AHF Analysentechnik did a good job for us
>(www.ahf.de). In my experience, major players like Chroma don't even bother
>to respond to custom filter requests - and I have been told of similar
>experiences. I am just saying because it might save you some time.
>
>Best regards,
>
>Kevin
>
>Kevin Braeckmans, Ph.D.
>Lab. General Biochemistry & Physical Pharmacy
>Ghent University
>Harelbekestraat 72
>9000 Ghent
>Belgium
>Tel: +32 (0)9 264.80.78
>Fax: +32 (0)9 264.81.89
>E-mail: [log in to unmask]
>
>
>
>
>>-----Oorspronkelijk bericht-----
>>Van: Confocal Microscopy List
>>[mailto:[log in to unmask]] Namens Guy Cox
>>Verzonden: dinsdag 13 maart 2007 5:15
>>Aan: [log in to unmask]
>>Onderwerp: Re: lasers for LSM
>>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>The efficiency of exciting fluorochromes is only part of it.
>>473 will be less good than 488 at exciting FITC but the
>>window for detecting FITC will be much wider with the
>>combination Maria proposes, so in the long run she'll
>>probably do better.
>>That does depend on a custom triple dichroic being available
>>for the 473, 559 and 635 combination.
>>Without that there will be a problem ...
>>
>> Guy
>>
>>
>>
>>
>>
>>>Hi Maria,
>>>If you go to either of these sites, you can see where common laser
>>>lines intersect the the spectra of fluorescent dyes and
>>>
>>>
>>proteins, and
>>
>>
>>>hence their efficiency. This, of course, does not take
>>>
>>>
>>into account
>>
>>
>>>the power output of the lasers.
>>>
>>>http://www.mcb.arizona.edu/IPC/spectra_page.htm
>>>
>>>http://www.mcb.arizona.edu/ipc/fret/
>>>Cheers,
>>>Carl
>>>
>>>Carl A. Boswell, Ph.D.
>>>Molecular and Cellular Biology
>>>University of Arizona
>>>520-954-7053
>>>FAX 520-621-3709
>>>----- Original Message -----
>>>From: "Maria Jimena Ortega" <[log in to unmask]>
>>>To: <[log in to unmask]>
>>>Sent: Monday, March 12, 2007 2:30 PM
>>>Subject: lasers for LSM
>>>
>>>
>>>Search the CONFOCAL archive at
>>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>>
>>>Hi,
>>>we use a system with 488, 543 and 633 lasers for common
>>>
>>>
>>fluorochromes.
>>
>>
>>>We are thinking of changing to a system with the 473, 559 and 635
>>>diodes lines.
>>>Does anyone have a comparison of the eficiency of this
>>>
>>>
>>lines for most
>>
>>
>>>used fluorochromes?
>>>
>>>Thanks a lot
>>>
>>>Maria
>>>
>>>
>>>
>>--
>>Associate Professor Guy Cox
>>Electron Microscope Unit,
>>University of Sydney,
>>NSW 2006, Australia
>>
>>Phone:+61 2 9351 3176 Fax:+61 2 9351 7682
>>http://www.guycox.net
>>
>>
>>
|
