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March 2007

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From:
Vasseur Monique <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 14 Mar 2007 10:53:50 -0400
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I would be very glad to get a pdf copy as well.  Thanks a lot.

Monique Vasseur
Microscopie et imagerie
Département de biochimie
Université de Montréal
C.P. 6128, succursale Centre-ville
Montréal QC    H3C 3J7   Canada
tél. (514) 343-6111 poste 5148
-----Message d'origine-----
De : Confocal Microscopy List [mailto:[log in to unmask]] De la part de Robert Zucker
Envoyé : 13 mars 2007 12:25
À : [log in to unmask]
Objet : Spectral Imaging beads

Molecular Probes has produced 8 Focal check spectral beads with 4 pairs
of fluorophores.  Each pair has wavelength maxima that are separated by
13 to 36 nm between 500 and 700 nm.

These beads can be used to evaluate the spectral reproducibility of
confocal systems such as the Nikon C1Si, Olympus FV1000, Leica SP and
Zeiss Meta 510; and non confocal systems such as PARISS, SKY and CRI.
They can also be used for spectral mixing studies.

We have recently published a paper in Cytometry entitled " Reliability
of Confocal Microscopy Spectral Imaging Systems: Use of Multispectral
beads"  Cytometry 71A 174-189 2007 which describes some application of
these beads to test various spectral imaging systems.

A PDF of the paper is available on request

Best wishes
Bob

Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory
Reproductive Toxicology Division, MD 67
2525 E.NC Highway 54
Research Triangle Park, North Carolina, 27711
Tel: 919-541-1585; fax 919-541-4017
e-mail: [log in to unmask]

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