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My experience in cultured CV1 cells using confocal imaging is that the
intensity of untagged XFP in the nucleus is the same as that in the
cytoplasm. In wide-field it appears to accumulate because the nucleus is
thicker than the cytoplasm as the cell spreads. Tagging the molecule so it is
bigger than 40-45kDa results in exclusion from the nucleus in both imaging
modes.

Richard C. Kurten Ph.D.
Associate Professor, Physiology & Biophysics
   UAMS College of Medicine
Co-Director, Lung Cell Biology Laboratory
   Arkansas Children's Hospital Research Institute
Director, Digital and Confocal Microscopy Laboratory
   Arkansas Cancer Research Center
Little Rock, Arkansas
UAMS: 501-686-8269 ACHRI: 501-364-2823


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of F Javier Diez Guerra
Sent: Friday, March 16, 2007 11:09 AM
To: [log in to unmask]
Subject: GFP in nuclei

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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi,

Anybody knows why EGFP (or any of its color
variants) concentrates at cell nuclei when expressed in eukaryotic cells?

I mean just EGFP, without any tag or fusion.

My experience with XFPs derived from A. Victoria is that they show
accumulation at cell nuclei.

Thanks


F Javier Diez-Guerra, PhD
Profesor Titular
Centro de Biologia Molecular Severo Ochoa Facultad de Ciencias, Universidad
Autónoma Ctra Colmenar Viejo Km 15 Cantoblanco, 28049 Madrid SPAIN

phone:  +34 91 4978051
Fax:      +34 91 4978087
e-mail: [log in to unmask] 

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