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Richard,

We use NIH-3T3 cells and see more concentration 
(intensity) of XFPs within nuclei using confocal imaging.

Further, we do not see differences between 
monomeric (A206K) and non-monomeric versions. All them concentrate at nuclei.


At 17:30 16/03/2007, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>My experience in cultured CV1 cells using confocal imaging is that the
>intensity of untagged XFP in the nucleus is the same as that in the
>cytoplasm. In wide-field it appears to accumulate because the nucleus is
>thicker than the cytoplasm as the cell spreads. Tagging the molecule so it is
>bigger than 40-45kDa results in exclusion from the nucleus in both imaging
>modes.
>
>Richard C. Kurten Ph.D.
>Associate Professor, Physiology & Biophysics
>    UAMS College of Medicine
>Co-Director, Lung Cell Biology Laboratory
>    Arkansas Children's Hospital Research Institute
>Director, Digital and Confocal Microscopy Laboratory
>    Arkansas Cancer Research Center
>Little Rock, Arkansas
>UAMS: 501-686-8269 ACHRI: 501-364-2823
>
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[log in to unmask]] On
>Behalf Of F Javier Diez Guerra
>Sent: Friday, March 16, 2007 11:09 AM
>To: [log in to unmask]
>Subject: GFP in nuclei
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi,
>
>Anybody knows why EGFP (or any of its color
>variants) concentrates at cell nuclei when expressed in eukaryotic cells?
>
>I mean just EGFP, without any tag or fusion.
>
>My experience with XFPs derived from A. Victoria is that they show
>accumulation at cell nuclei.
>
>Thanks
>
>
>F Javier Diez-Guerra, PhD
>Profesor Titular
>Centro de Biologia Molecular Severo Ochoa Facultad de Ciencias, Universidad
>Autónoma Ctra Colmenar Viejo Km 15 Cantoblanco, 28049 Madrid SPAIN
>
>phone:  +34 91 4978051
>Fax:      +34 91 4978087
>e-mail: [log in to unmask]
>
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F Javier Diez-Guerra, PhD
Profesor Titular
Centro de Biologia Molecular Severo Ochoa
Facultad de Ciencias, Universidad Autónoma
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN

phone:  +34 91 4978051
Fax:      +34 91 4978087
e-mail: [log in to unmask]