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I used cell stored at -80 that were more than 1 year old without
problem. There is indeed a decrease in viability but nothing that
persisted for more than one split. However it is a well known fact that
a canister full of liquid nitrogen is less likely to fail during a power
shortage or having a compressor malfunction. 

Gabriel

On Fri, 2007-20-04 at 12:50 -0500, Prabhakar Pandian wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hello,
>       Can anyone tell me how long one can store mammalian cells in a -80
> freezer without the use of liquid nitrogen.
> 
> We are looking for a short term storage (3-6 months) for continuous use of
> primary cells and cells lines, but don't have access to liquid nitrogen
> storage. Hence this solution of freezing batches in  -80 freezer.
> 
> Also does it make a difference using whether a -80 or a -86 freezer.
> 
> 
> Thanks,
> 
> -Prabhakar
>