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Dear Vitaly,

I just received your P.S. Were you kidding? I did start a reply about 
using 2-D electron diffraction quality crystals, surface packing and 
orientation problems, and the effects of surface charge and surface 
polarity effects on dipole orientation. Some of these factors would 
affect quantum efficiency, intermolecular quenching, etc. even if you 
meant um and not nm.

So joke or not, what is the radius of the GFP construct you were 
considering? I have conjugated fluorescent antibodies to micron sized 
lithographically produced silicon particles.

Anyway....


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear List,
>
>I would greatly appreciate any reference to the study on the 
>intermolecular self-quenching of commonly used fluorescent proteins, 
>e.g. CFP, GFP, YFP, mRFP1 etc.
>The proteins are expected to be immobilized on the glass surface at 
>different spacing intervals (1 nm, 2 nm, 3 nm, 4 nm apart and so on).
>
>Thank you in advance,
>
>Vitaly
>
>NCI-Frederick,
>301-846-6575


-- 
________________________________________________________________________________
Mario M. Moronne, Ph.D.
ph (510) 528-2400

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