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Hi,

I wander what the x-y resolution is when the pixel size is the limiting factor?
Is it 2-3 times the pixel size in x-y?

What is the resolution along the z-axis if I collect image stacks?

I do low magnification imaging of mice with a laser scanning 
microscope using a 4x objective with N.A of 0.16 and  wavelength 
around 650 nm.
Pixel size is 5.4 micro meter because we need the large field of view.
Airy disc size is about 2.5 micro meter.

Rainer





-- 
Rainer Kohler, Ph.D
Microscopist
Center for Molecular Imaging Research
Massachusetts General Hospital/ Harvard Medical School
Building 149, 13th  Street, Room 5406
Charlestown, MA 02129-2060
Tel. 617 726 5788
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