Search the CONFOCAL archive at
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Scott,

Jim Pawley's "The 39 Steps: A Cautionary Tale of Quantitative 3-D 
Fluorescence Microscopy"
http://www.zoology.wisc.edu/faculty/Paw/pdfs/The_39_Steps_corrected.pdf

Allison J. North's "Seeing is believing? A beginners’ guide to practical 
pitfalls in image acquisition"
http://www.jcb.org/cgi/reprint/172/1/9

Are good places to start. Please note that I am of the opinion that 
real, scientifically performed quantitative fluorescence, is rarely 
done. When I point out to people the number of things that they need to 
accurately account for to accomplish quantitative fluorescence most of 
them back down.

Doug

Scott Howell wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear List,
>
>
> I have a lab with very little microscopy experience wanting to start 
> doing some quantitative fluorescent measurements on their proteins of 
> interest. Does anyone know of a good article (not to technical) that I 
> can have them read to begin to get them up to speed?? Just looking for 
> a decent "for beginners" article. Thanks. 
>
> Scott J. Howell, Ph.D.
> Manager, Imaging Module
> Visual Sciences Research Center
> Case Western Reserve University
> Institute of Pathology Room 106
> 216-368-2300
> http://www.case.edu/med/vsrc/
>
>   

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Douglas W. Cromey, M.S. - Assistant Scientific Investigator
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