Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

ImageJ has quite a few very nice plugins for colocalization.  BTW, I 
also have a macro for ImageJ that converts a red/green color image 
(i.e FITC/RITC) to magenta/green, for deuteranopes like me (and you).

Date sent:      	Mon, 14 May 2007 10:37:28 -0500
Send reply to:  	Confocal Microscopy List 
<[log in to unmask]>
From:           	Andrew Resnick <[log in to unmask]>
Subject:        	Colocalization
To:             	[log in to unmask]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> We have several image processing programs (MetaMorph, ImageJ, etc.), 
> and I have been trying to help the users of our Core who consistently 
> perform colocalization studies. Right now, colocalization is 
> determined by simply overlaying the (usually red and green) images 
> and looking for yellow.  This is not suitable for color-blind users 
> (including myself).  I would like to have the colocalization routine 
> simply replace the pixel color with a user-defined color (say white 
> or bright pink) if the threshold for colocalization is over a 
> user-defined limit.  Does that make sense?
> 
> I'm not an expert user of either MM or ImageJ, so if there is a 
> plugin for either, that would be great. Any hints or tips would be appreciated.
> 
> Andy
> 
> Andrew Resnick, Ph. D.
> Instructor
> Department of Physiology and Biophysics
> Case Western Reserve University
> 216-368-6899 (V)
> 216-368-4223 (F) 


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Joel B. Sheffield, Ph.D.
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