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For the lifetime imaging you could always use the Nikon
(Europe) LIMO system - that is available as a stand-alone
unit for mounting on non-Nikon scanners.  (They supply
appropriate software - of course this will not be
integrated into the control software the way it is on a
Nikon scanner).

I don't doubt that the Becker & Hickl will be able to
be fitted to an Olympus, but I've only used it on other
scopes.   Picoquant also make a system.

As for the spectral issue, if you just want to 'park'
the beam and read a spectrum from one point a simple
spectrometer such as Ocean Optics could be fibre-linked
to your signal but sensitivity might be an issue.  I
use one regularly in widefield but that's integrating a
larger area.

                                            Guy



> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear confocalists,
>
>  We are writing a grant proposal to support a research project that aims
> to
> study some key molecules in oncogenesis through FRET with respect to cell
> cycle. We see a need for certain equipment and would like to know people's
> experience with what equipment serve the purpose, are cost effective and
> are
> configurable. I would like to include quotes for the equipment also in the
> grant proposal, so commercial vendors please get in touch off-list. Please
> note that we are from singapore.
>
> We have an Olympus fluoview 300 based on IX71 (inverted) system with
> two-photon laser (Coherent MIRA 900F) + 488nm and 543nm lasers. FV300
> looks
> like an old system, but it allows us to modify the light path when in
> need.
> We plan to outfit it with following :
> 1. A spectrometer coupled to scan head so that we can obtain spectral data
> per pixel.
> I have seen Zeiss 510 META system with MCS 510 spectrometer that provides
> spectral data at 2 nm resolution per pixel.  Zeiss supplies a macro to (I
> guess) register the spectral data as a function of pixel position.
> Anything
> similar possible or existing with FV300 also?
>
> 2. A TCSPC system. I think Becker-hickel is the right place. But does any
> one have suggestions on reasonably priced but working solution with FV300?
>
> Thanks in advance for all inputs,
>
> Best regards,
> Shalin
>
> --
> My co-ordinates:
> Shalin Mehta, Graduate student
> Graduate Programme in Bioengineering, NUS, Singapore
> Email: shalin {dot} mehta {at} gmail {dot} com
> Blog: electricsbm.blogspot.com
> Mobile: +65 90694182
>
> "Hindsight is 20/20"
>


-- 
Associate Professor Guy Cox
Electron Microscope Unit,
University of Sydney,
NSW 2006, Australia

Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
http://www.guycox.net