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Dear List,

 I have obtained pRSET-B mcherry plasmid from another lab. With help
of biologist friend, I am trying to sub-clone mCherry into another
plasmid (pCDNA3) such that we can express fusion of our protein and
mCherry. How do people find mCherry fusions work
properly?Protein-mCherry or mCerry-protein and does it necessarily
need linker?

Also, I don't understand most of the terms in plasmid map (as of now I
have taken only one course in molecular biology). Could anyone please
help me expand them?

pUC - is it a promoter? If yes, can it be used to express mcherry
protein in colon cancer cells as a control for the fusion?
PT7
RBS
Xpress
Epitope
EK

This is the link to the plasmid map:
http://www.tsienlab.ucsd.edu/SUBWEB%20-%20Samples/PDF/mCherry-map%20&%20sequence.pdf

Thanks very much,
regards,
Shalin

-- 
My co-ordinates:
Shalin Mehta, Graduate student
Graduate Programme in Bioengineering, NUS, Singapore
Email: shalin {dot} mehta {at} gmail {dot} com
Blog: electricsbm.blogspot.com
Mobile: +65 90694182