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July 2007

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Fri, 6 Jul 2007 11:36:47 -0400
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Confocal Microscopy List <[log in to unmask]>
Subject:
Curvature in YZ plane
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Confocal Microscopy List <[log in to unmask]>
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Sarah Kefayati <[log in to unmask]>
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Hello every one,

It's about two weeks that I've started imaging Tetrascpeck
beads(.2micron),I see something very strange when I do the sectioning:

looking at YZ plane, I see the curvy shape ,it's like there is a
bending point.I thought maybe it's because of astigmatism of our diode
laser so I switched the laser with Ti-sapphire ,I still can see the
curvature in yz plane.my second guess is stage displacement in y
direction during sectioning in z direction,or maybe it's an aberration
with our objective lens(we're using Olympus 60x/1.2 water immersion
objective)...

I appreciate if you have any information regarding this problem and
share with me.

regards
sarah

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