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July 2007

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Date:
Thu, 12 Jul 2007 13:47:55 +0200
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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Dendra2
From:
David Stanek <[log in to unmask]>
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Elisa,

We tagged nuclear proteins with Dendra2 and expression in  human cell  
culture is nice . The only problem is that we can't get  
photoconversion using 488nm or 458nm laser lines and have to use  
405nm diode laser. Then it works well.

Regards
David

On Jul 11, 2007, at 4:31 PM, BIC-UKN wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Community,
> has anyone experience with the new photoswitchable FP from Evrogen,  
> Dendra2? Is it expressed nicely and how efficient is photactivation  
> with blue light?
> Thanks
> Elisa

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