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Ammasi Periasamy wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hello
> 
> We tried the mOrange with eYFP, it works ok, not great. Most of the red 
> proteins are not working good for FRET, do not know why. Some one tried 
> mCherry, it did not work. If I get more info, I will put it in the list 
> server.
> 
I have measured 30-40% FRET efficiency with a YFP-mRFP1 fusion, using the 
acceptor photobleaching method. Given the minimum distance between the 
fluorophores which is imposed by the protein structure, and the likelyhood that 
the alignment of the dipoles is not perfect,  I would not expect more.

--aryeh
-- 
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-7384050