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Dear Caroline,

We use the 465nm LED Array Modules of the precisExcite product from
CoolLED Ltd in combination with a regular EGFP filterset from Chroma
with good success. They state their 465nm LAM offers greater power than
your 100W mercury bulb, which is well possible, because the Hg arc has
no peak around this region.

No commercial interest.

regards, jens
 
---
Dr. Jens Rietdorf 
Head Microscopy
Novartis Research Foundation
Friedrich-Miescher-Institute, wro1066.2.32
Maulbeerstr.66, CH-4058 Basel, Switzerland
phone +41(61)69-75172 mobil +41 798284737
Email:[log in to unmask]

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Caroline Bass
Sent: Freitag, 20. Juli 2007 06:03
To: [log in to unmask]
Subject: Student EGFP scope

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Hello,

Sorry to ask such a low tech question, but has anyone tried or have
opinions about blue LED adapted scopes for visualizing EGFP?  I have
gotten fairly good results from large tissue blocks expressing EGFP
using a strong blue LED flashlight, but I don't know how feasible it is
for sections.  Here are two links for do-it-yourself guides.

http://www.plantsci.cam.ac.uk/Haseloff/imaging/cheaposcope/cheaposcope.h
tm

http://130.15.90.245/gfp_stereoscope.htm

In particular I am thinking about adapting a dissecting microscope for
very gross visualization of EGFP in brain sections.  The sections will
probably be from 50 microns to 1 mm.  Does anyone have experience with
this or suggestions?  

Thanks,

Caroline