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Shalin Mehta <[log in to unmask]> writes:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=
> confocal Hello Sarah,
>
>  The emission spectrum in 2-p case will be the same as 1-p case. I am sending
> the TIFF to you. Interestingly, when I used the beads with 2-p excitation I
> found that they melted when I tried to acquire experimental PSF in blue
> region. They seem to be poor emitters in blue region. Additionally, they emit
> the signal in blue,green, red region (with increasing intensity in that order)
> - it is a tell-tale sign that they are made up of QDs.

It would really surprise me if Tetra-speck beads were quantum dots. I
have used them for about 10 years, and 10 years ago QDs were basically
non-existent.

Ian