Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

What's about the 438 nm line? Has anyone tested the Dendra2 with?

Vitaly

----- Original Message ----- 
From: "David Stanek" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Thursday, July 12, 2007 6:47 AM
Subject: Re: Dendra2


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hi Elisa,
> 
> We tagged nuclear proteins with Dendra2 and expression in  human cell  
> culture is nice . The only problem is that we can't get  
> photoconversion using 488nm or 458nm laser lines and have to use  
> 405nm diode laser. Then it works well.
> 
> Regards
> David
> 
> On Jul 11, 2007, at 4:31 PM, BIC-UKN wrote:
> 
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear Community,
>> has anyone experience with the new photoswitchable FP from Evrogen,  
>> Dendra2? Is it expressed nicely and how efficient is photactivation  
>> with blue light?
>> Thanks
>> Elisa
>