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July 2007

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From:
Renato Mortara <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 16 Jul 2007 12:35:39 -0300
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Hi there,

I have a collaborator that is a pathologist involved in tropical diseases
(Leishmaniasis and Chagas' disease) and he has several slides of tissues
labeled for collagen fibers (using the Masson stain that renders the fibers
blue/green) and reticular fibers (stained with Gomori ammonium-silver) that
stains these fibers black. He wished to image the stains and counter-stains
by confocal microscopy.

I hava a BioRad 1024 UV confocal with na Argon UV laser (353/361, 488 and
514 nm lines) and a Kr/Ar (488/568/647 nm lines). 

Has anyone some hints as to how (exc/emm lines/filters, etc.) - and if it is
possible to image such stains ? 

Any inputs are most welcome !

Renato

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