Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi there,
I have a collaborator that is a pathologist involved in tropical diseases
(Leishmaniasis and Chagas' disease) and he has several slides of tissues
labeled for collagen fibers (using the Masson stain that renders the fibers
blue/green) and reticular fibers (stained with Gomori ammonium-silver) that
stains these fibers black. He wished to image the stains and counter-stains
by confocal microscopy.
I hava a BioRad 1024 UV confocal with na Argon UV laser (353/361, 488 and
514 nm lines) and a Kr/Ar (488/568/647 nm lines).
Has anyone some hints as to how (exc/emm lines/filters, etc.) - and if it is
possible to image such stains ?
Any inputs are most welcome !
Renato