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Hi again,
I just realised that when I mentioned about the humidified gas coming up to the working head, it may not be clear what I meant for those who aren't familiar with these Solent systems. The humidified gas in comes through narrow tubing up to the specimen rather than filling the entire box.
Kind regards,
Jacqui
Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND
Tel: 64 9 373 7599 Ext 87438
Fax: 64 9 373 7484
http://www.health.auckland.ac.nz/biru/
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Jacqui Ross
Sent: 15 November 2007 13:04
To: [log in to unmask]
Subject: Re: Microscope Stage Incubators
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Charles & others,
We have Solent incubation (box-style) systems on two microscope systems, one an Olympus FV1000 confocal microscope and the other a Nikon TE2000E inverted microscope. We've had both systems for around 2 years without obvious rust issues at the moment. Both systems are multi-user.
With these systems, the gas is humidified but it comes up to the working head of the microscope and of course your specimen is bathed in liquid but it's still a fairly small volume in our case.
I've never noticed condensation dripping off the sides of the box and since it's not completely sealed, moisture does have a chance to dry out. In fact, the real risk of rust comes with the use of the dipping objectives on our confocal, which drip down buffer salts, etc. onto the condenser, etc. So it is important to clean that off to avoid rust.
The reason we bought a box-style system for our inverted microscope was because when I had a look at all the available chamber-based systems, there seemed to be variable reports about how well they worked especially since we operate as a multi-user facility.
Our systems are also used by people who don't need the incubation system but because we have the box structure rather than a chamber system, we don't have to keep taking things off and on.
One other concern was that you seemed to need to buy other components such as objective heaters, etc. to avoid heat sinks/focus drift if you have a stage-top incubator whereas with the box-style, more of the system is heated and as long as you give sufficient time for things to equilibrate, I think it works quite well.
However, auto-focus (as mentioned previously by Gabor) is still a useful thing to have with a box-style system.
Kind regards,
Jacqui
Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND
Tel: 64 9 373 7599 Ext 87438
Fax: 64 9 373 7484
http://www.health.auckland.ac.nz/biru/
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Chris Wood
Sent: 15 November 2007 11:44
To: [log in to unmask]
Subject: Re: Microscope Stage Incubators
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
So is this scaremongering or real? Has anybody on the list ever had a
microscope degrade/die/rust to smithereens because they coupled it to an
box-style humidified chamber?
And has anyone ever used the Bioptechs rebuilding service for these
reasons, and if so, what was found?
Saludos
Chris
Dr Chris Wood
Instituto de Biotecnología
Universidad Nacional Autónoma de México
Av. Universidad 2001
Col. Chamilpa
Cuernavaca 62150
Morelos
México
On Tue, 13 Nov 2007 21:54:09 -0500, George McNamara
<[log in to unmask]> wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi Charles,
>
>Check out the Precautionary Note section of the web page
>http://www.bioptechs.com/Products/Rebuild/rebuilding_service.html
>
>
>
>
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