Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Tino Jäger schrieb:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I want to ask, if anyone is experienced in triggering the Leica 
> Confocal Software (LCS).
>
> We want to trigger a Leica SP2 with the heartbeat of a rat during 
> image acquisition.
>
> Every single frame during a scan should be triggered. We image for ~ 2 
> min, one frame every 400 ms (~ 2 Hz). During the 2 min data 
> acquisition the heartbeat rate changes and therefore every single 
> frame needs to be triggered (not only the beginning of data acquisition).
>
> Does anyone know answers to the following questions?
>
> How long is the delay between the trigger input and scan start (start 
> of data acquisition)?
> More important, is this delay constant or variable during the 
> acquisition period?
> We tried to trigger the laser with 1 Hz, defining one job in the life 
> data mode (time interval 0, frames 1, next job: job 1 – rerun) but the 
> software crashes.
> (life data mode; input: TTL-pulses (5 V, 20 ms); acquisition: frame 
> size 512 x 128; 400 Hz; bidirectional scan; xyt – recordings)
> Moreover, every job creates one image. Is there a possibility to 
> combine them after every acquisition?
>
> Thanks for your help!
> Tino
>

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HEIMO WOLINSKI, PhD

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