Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I think it depends how you want to trigger the software. Do you want to 
do it by the COM interface? We control the LCS this way for acquiring 
mosaic image data. The response time seems to be quite constant but I 
don't know exact timings. If you are interested, we could do some timing 
experiments.

I'm afraid, the LCS is not stable in any case if triggered by COM. It 
depends highly on the version and the exact use case if an experiment 
runs reliable...

Best regards,
Christian

Tino Jäger schrieb:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> I want to ask, if anyone is experienced in triggering the Leica Confocal 
> Software (LCS).
> 
> We want to trigger a Leica SP2 with the heartbeat of a rat during image 
> acquisition.
> 
> Every single frame during a scan should be triggered. We image for ~ 2 
> min, one frame every 400 ms (~ 2 Hz). During the 2 min data acquisition 
> the heartbeat rate changes and therefore every single frame needs to be 
> triggered (not only the beginning of data acquisition).
> 
> Does anyone know answers to the following questions?
> 
> How long is the delay between the trigger input and scan start (start of 
> data acquisition)?
> More important, is this delay constant or variable during the 
> acquisition period?
> We tried to trigger the laser with 1 Hz, defining one job in the life 
> data mode (time interval 0, frames 1, next job: job 1 – rerun) but the 
> software crashes.
> (life data mode; input: TTL-pulses (5 V, 20 ms); acquisition: frame size 
> 512 x 128; 400 Hz; bidirectional scan; xyt – recordings)
> Moreover, every job creates one image. Is there a possibility to combine 
> them after every acquisition?
> 
> Thanks for your help!
> Tino
> 


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