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Just to add that, line accumulation is available since LAS AF v1.8. So 
you can finally set it up using "Live" Scan.

How does 2x Accumulation compete compared to 2x more laser power? The 
total illumination power added to one pixel is in the end simliar. So 
signal-to-noise and bleaching effects should be comparable as well, or not?

cheers,
Michael


Stefan Terjung wrote:
> - But you can get quite comparable time-lapse sequences with a bit 
>   more 'fine-tuning' on the SP5 (at least for some cases):
> 	a) use accumulation (unfortunately only frame accumulation 
>          available yet) to compensate for lower brightness due to 
> 	   shorter pixel dwell time instead of increasing the laser power 
> 	   => this is similar to increasing the exposure time on a 
>            spinning disk without saturating the fluorophores