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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

But how do you de-scan in the transmission beam path? You would need a 
second scanner behind the condenser which has to be synchronized with 
the main one, to bring the light to the pinhole.

cheers,
Michael


John Oreopoulos wrote:
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal This is a good 
> question and I've wondered why the commercial systems don't have 
> a transmission pinhole as well. I'm sure it must be possible because I 
> think Minski's original confocal microscope had a transmission optical 
> design. It's possible to form reflection confocal images with the 
> backscattered laser light if you remove the emission filters in front of 
> your detectors, and there isn't any tricky pinhole alignment associated 
> with that. Perhaps that is the main reason - a transmission pathway 
> pinhole would have to be aligned to the system and this might be 
> difficult to maintain. In the epi configuration, the objective also acts 
> as the condenser, whereas in the transmission pathway you have to align 
> the objective, the condenser, and a pinhole.
> 
> 
> John Oreopoulos, BSc,
> PhD Candidate
> University of Toronto
> Institute For Biomaterials and Biomedical Engineering
> Centre For Studies in Molecular Imaging
>  
> 
> On 6-Nov-07, at 10:03 AM, John Runions wrote:
> 
>> Search the CONFOCAL archive at 
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal 
>> Hello folks,
>>
>> Here is one of those seemingly straightforward to answer questions 
>> that has me really stumped.  You should hear all of the BS around here 
>> when I bring this up with the other confocal 'experts'.  Why isn't 
>> there a pinhole in the transmission-image forming pathway?  A confocal 
>> transmission image would be nice but I always tell people it's not 
>> possible.  Is it not possible or just not done?
>>
>> Thanks for your help.  John.
>> -- 
>>
>> *********************************
>> C. John Runions, Ph.D.
>> School of Life Sciences
>> Oxford Brookes University
>> Oxford, UK
>> OX3 0BP
>>
>> email:  [log in to unmask] <mailto:[log in to unmask]>
>> phone: +44 (0) 1865 483 964
>>
>> web: http://www.brookes.ac.uk/lifesci/runions/HTMLpages/index.html! 
>> <http://www.brookes.ac.uk/lifesci/runions/HTMLpages/index.html%21>
>>
>>  
>>
>>
>> New - Oxford Brookes Master's in Bioimaging with Molecular Technology 
>> <http://www.brookes.ac.uk/studying/courses/postgraduate/2007/bmt>
>>
>>
> 
> 
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> 
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