Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Sivaguru, I would like to suggest you to go through the site http://www.microscopy-uk.org.uk/primer/basics.htm http://www.microscopyu.com/ http://www.bio.uci.edu/academic/grad/Basic%20Training/Microscopy1_files/frame.htm http://science.howstuffworks.com/light-microscope1.htm In addition, I shall send some files to you by mail. Regards, Gene Maverick, Application Specialist Nikon Cell and Molecular Imaging Group.