CONFOCALMICROSCOPY Archives

February 2008

CONFOCALMICROSCOPY@LISTS.UMN.EDU

Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
From:
"E. Tim O'Brien" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 15 Feb 2008 10:21:23 -0500
Content-Type:
text/plain
Parts/Attachments:
text/plain (35 lines)
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Not sure if this point has been made, but we have a CSU-10, and I have a 
cheap, cooled camera on it.  I constantly wish I had a faster, more 
sensitive camera on it.  An EMCCD would be ideal. The confocal is fast 
but not that much light comes through to your sample.  So a cheap camera 
(which will be slow) may ultimately be frustrating, and would not 
capitalize on the speed of the spinning disk. An EMCCD (512 x 512) can 
easily capture at 30 FPS with very good sensitivity, while a normal 
camera, like a COOLSnap, etc, might be able to do 5 or 10 FPS.  The 
benefits increase for GFP in living cells, since high sensitivity means 
less light is necessary and thus less photobleaching. 

So I would maybe get a used camera for the time being, and save up for 
an EMCCD for that application. (Which I am trying to do myself!)

Thanks,
Tim O'Brien
UNC Physics



Collin White wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
> I have a CSU-10 spinning disc confocal unit and want to hook it to the 
> cheapest high res camera possible. Any ideas/opinions from all would 
> be appreciated.
> Thank you in advance!
> Collin
>

ATOM RSS1 RSS2