Subject: | |
From: | |
Reply To: | |
Date: | Fri, 15 Feb 2008 10:21:23 -0500 |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Not sure if this point has been made, but we have a CSU-10, and I have a
cheap, cooled camera on it. I constantly wish I had a faster, more
sensitive camera on it. An EMCCD would be ideal. The confocal is fast
but not that much light comes through to your sample. So a cheap camera
(which will be slow) may ultimately be frustrating, and would not
capitalize on the speed of the spinning disk. An EMCCD (512 x 512) can
easily capture at 30 FPS with very good sensitivity, while a normal
camera, like a COOLSnap, etc, might be able to do 5 or 10 FPS. The
benefits increase for GFP in living cells, since high sensitivity means
less light is necessary and thus less photobleaching.
So I would maybe get a used camera for the time being, and save up for
an EMCCD for that application. (Which I am trying to do myself!)
Thanks,
Tim O'Brien
UNC Physics
Collin White wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
> I have a CSU-10 spinning disc confocal unit and want to hook it to the
> cheapest high res camera possible. Any ideas/opinions from all would
> be appreciated.
> Thank you in advance!
> Collin
>
|
|
|