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Hi Tomo,

Our university biological safety officer requires that all pathogenic
specimens be contained. Therefore, we always have these types of
specimens under a sealed coverslip, i.e. no direct contact with the
objective lens, etc. Obviously, this isn't perfect but it does minimise
the risks. We do have a few people imaging biofilms in this way.

I should mention that at the moment, all our confocal systems are on
upright stands.

Kind regards,

Jacqui 


Jacqueline Ross 

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences 
The University of Auckland 
Private Bag 92019 
Auckland, NEW ZEALAND 

Tel: 64 9 373 7599 Ext 87438 
Fax: 64 9 373 7484 

http://www.health.auckland.ac.nz/biru/ 


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Tomohiro Kawaguchi
Sent: Monday, 18 February 2008 3:52 p.m.
To: [log in to unmask]
Subject: Biosafety

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Hi! All,

We have one user who wants to set up the flow cell to image live
pseudomonas aeruginosa biofilm using our confocal microscope (multi-user
confocal microscope open for our university community).

This user said that p.aeruginosa is not so dangerous as long as we are
careful and clean them up after its use.  I want to get a biosafety
officer involved before we do anything.

If you can share your experience on biosafety regarding live pathogen in
your confocal room, I really appreciate it.

Many thanks!

Tomo Kawaguchi