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Sarah,
Check out the SVI website, www.svi.nl or contact them for information  
on their 2D deconvolution. there are some tips regarding setting the  
z-thickness, etc.  a 2D is treated as a seriously truncated volume  
and will use a PSF.  I would think that if the PSF is similarly  
truncated, then a calculated PSF might be more accurate.  In case of  
the latter, you may need to measure the refractive index of your  
samples.
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[log in to unmask]

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>


>
> Sarah Kefayati wrote:
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hello all:
>>
>> I am collecting in situ psfs from Arabdiopsis leaves.For me it is
>> impossible to collect PSF's in z direction  as I will also capture  
>> the
>> leaf structure too and I only collect data in XY plane.
>> I am wondering if it is possible to do 2D deconvolution by these
>> collected PSF s with Huygens software(we haven't get the soft ware  
>> yet
>> we would like to try this with the test licence of the software).
>>
>> I really appreciate any information and any idea.
>>
>> Sarah
>>
>>
>
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