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March 2008

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Fri, 14 Mar 2008 09:13:14 +1100
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Confocal Microscopy List <[log in to unmask]>
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Matt Burton <[log in to unmask]>
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Benoît,

the filters are the same, there should be no problem using them. Note the 
15.8 mm dichroic mirrors used in a LSR II have an AOI of 11.25 degrees not 
the standard 45.

Matt

At 01:51 PM 13/03/2008 Thursday, you wrote:
>Search the CONFOCAL archive at 
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello, flowers and 
>microscopists!
>
>Here is a chimera-type question!
>
>We have emission filters from an BD LSRII flow cytometer (disks of 25mm of 
>diameter) and would like to use them in a CSU-X1 confocal head. Basically, 
>the filters are fitting in the emission wheel of the CSU and we see 
>fluorescence. My question is: do emission filters for flow cytometry and 
>for confocal microscopy have the same properties?
>I may be wrong, but both are the final emission filters in front of either 
>the PMT or the camera and thus have a similar role of filtering the 
>emissions. In my mind, both filters should be comparable. Do I omit any 
>critical detail?
>
>Thank you!
>
>benoit
>
>--
>Benoît Labarthe, PharmD
>Institut de Cardiologie de Montréal
>Montreal Heart Institute
>Centre de Recherche, Laboratoire du Dr.Théroux
>5000 Bélanger Est
>Montréal Québec Canada H1T 1C8
>Tel (514)-376 3330 ext 3017
>Fax (514)- 376 1076

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