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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Benoît,
the filters are the same, there should be no problem using them. Note the
15.8 mm dichroic mirrors used in a LSR II have an AOI of 11.25 degrees not
the standard 45.
Matt
At 01:51 PM 13/03/2008 Thursday, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello, flowers and
>microscopists!
>
>Here is a chimera-type question!
>
>We have emission filters from an BD LSRII flow cytometer (disks of 25mm of
>diameter) and would like to use them in a CSU-X1 confocal head. Basically,
>the filters are fitting in the emission wheel of the CSU and we see
>fluorescence. My question is: do emission filters for flow cytometry and
>for confocal microscopy have the same properties?
>I may be wrong, but both are the final emission filters in front of either
>the PMT or the camera and thus have a similar role of filtering the
>emissions. In my mind, both filters should be comparable. Do I omit any
>critical detail?
>
>Thank you!
>
>benoit
>
>--
>Benoît Labarthe, PharmD
>Institut de Cardiologie de Montréal
>Montreal Heart Institute
>Centre de Recherche, Laboratoire du Dr.Théroux
>5000 Bélanger Est
>Montréal Québec Canada H1T 1C8
>Tel (514)-376 3330 ext 3017
>Fax (514)- 376 1076
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