Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
All of the Alexa fluor conjugates have a tendency to stick non-
specifically via charge interactions of the anionic dye moiety. the
Image-iT fx does helps greatly, especially in thick samples.
In regard to using the blue fluorophores for the more prominent
targets in the tissue, the human visual system is only weakly
responsive to blue wavelengths and lacks blue-sensitive cones in the
fovea, the region of greatest visual acuity. In general, if the
smallest or rarest bits get the green, red or gray channel, they are
noticeable with less histogram manipulation than if the smallest bits
are viewed in the blue channel.
Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[log in to unmask]
************************************************************************
******
The box said "Requires WindowsXP or better", so I bought a Macintosh.
************************************************************************
******
On Mar 6, 2008, at 7:09 AM, Junya HIROI wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Christophe,
>
> I was also looking for a fluorescent dye for 405 nm excitation, and
> I compared Alexa 405
> with Pacific Blue. Both dyes bleached to some extent with
> ProlongGold, but Pacific Blue was
> absolutely brighter than Alexa 405, when compared at the same
> dilution. So, I recommend
> Pacific Blue, although Molecular Probes sells Pacific Blue for flow
> cytometry.
>
> I found another problem on Alexa 405: it nonspecifically binds to
> nuclei ! I asked Molecular
> Probes/Invitrogen about this problem, and they recommended me to
> use their blocking
> solution, Image-iT FX Signal Enhancer. It actually worked well,
> but Pacific Blue does not have
> such a problem, so that is another reason I like Pacific Blue.
>
> Junya
>
> --
> _____________________________________
> 廣井準也
> 聖マリアンナ医科大学 解剖学教室
> 〒216-8511 川崎市宮前区菅生2-16-1
> tel: 044-977-8111 内線3629
> fax: 044-976-7083
> e-mail: [log in to unmask]
> _____________________________________
>
> --
|
