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Dear Steffen,

amongst many other commercial suppliers for heating equipment, EMBL sells
controllers or complete mic enclosures, and as far as I know the only system
to control CO2 in the entire mic enclosure. The system is meanwhile widely
used. I have been involved with the development of the system, please feel
free to contact me offline for more info (also, I declare a minimal
commercial interest, I receive some inverntor compensation, though almost
the entire money is reinvested into development of technology and support of
the workshops).

Concerning the objective heater solution, these put serious stress on the
objectives and the heated volume is small, so to produce a long term stable
situation, its not ideal. However, for fast heating and cooling, like for
example to switch on/off temp sensitve allels, these heaters are suitable.
EMBL has developed a device to heat/cool a small volume at 1degC per sec. 

enclosure heater+CO2
http://www.embl-em.de/products/index.php?c=4&n=2&p=1&id=159
fast response heater
http://www.embl-em.de/products/index.php?c=4&n=2&p=1&id=396

EMBLEM Technology Transfer GmbH
Boxbergring 107, D-69126 Heidelberg, Germany
Tel: +49 6221/363-2210
Fax: +49 6221/363-22 29
E-mail: [log in to unmask]
www.embl-em.de 
 
Cheers, jens 


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Steffen Steinert wrote:
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hello,
> 
> I´m going to build a heating stage/chamber in order to do live imaging at
> 37°C with the ordinary 35mm-dishes . Due to lack of experience with these
> systems are there any general advices about eventual pitfalls or what
> works
> perfectly fine/not? Are there eventually some instruction guidelines out
> there?
> 
> Since I´ve built a complete home-made scope, I´m not particularly limited
> in
> terms of space or compatibility to Zeiss, Olympus, Leica etc. Currently,
> I´ve two separate systems in mind:
> 
> 1)      A flexible system that separately warms the objective as well as
> the
> dish. 
> 
> -          Does that work efficiently or is the loss of heat too
> significant
> rendering this method rather meaningless?
> 
> -          Any recommendations where to get such warming equipment for
> reasonable prices?
> 
> 2)      A complete plexiglas chamber which could also be utilized for
> CO2-adjustment.
> 
> -          Are these incubation chambers so relatively big volume-wise
> because of trying to avoid significant flows which could disturb the
> sample?
> Or is it of other practical reasons?
> 
> -          What kind of heating source (ventilator) would you suggest, any
> particular recommendations?
> 
>  ........
> 
> 
> Steffen Steinert, Dipl.-Ing.
> 
>  
> 
> --------------------------------------
> 
> Universität Stuttgart
> 
> 3. Physikalisches Institut
> 
> Pfaffenwaldring 57
> 
> 70550 Stuttgart
> 
>  
> 
> Tel.: 049/0711/68569832
> 
> Fax:  049/0711/68565281
> 
>  
> 
> http://www.pi3.uni-stuttgart.de/en/
> 
> --------------------------------------
> 
>  
> 
> 
> 

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