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Michael Weber <[log in to unmask]> writes:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> The correct pixel size is just correct by chance, since the Zeiss software
> doesn't consider the pinhole diameter in the calculation of XY scaling.

There is some justification for this. Using the Rayleigh
criterion, the resolution is defined by the distance to the first
minimum of the Ariry disk. A confocal pinhole does not change this at
all, although it does reduce the FWHM of the peak (ie the shape
changes but the peak still has the same total width). Where a confocal
really wins is in Z resolution.

Ian