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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I have a simple question, related to an annoying problem.
I have an image of a multilabelled fluorescent specimen, displayed as a merged RGB image. I would like to analyze just the red channel, so I split the channels and obtain B & W images of the individual component. They all look as bright as the original.
However, if I only have a single red image and would like to convert it to a B & W image (change mode RGB to grey), for 8-bit analysis in ImageJ, for example, the resulting image is very dull and fainter. Furthermore, this effect is much more dramatic with a red image than with a green image.
What is happening?
Thanks,
Judy
Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph: 416-864-6060 x6337
pager: 416-685-9219
fax: 416-864-6043
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