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Dear all,
afaik one can combine the white laser with the standard laser module.
Don't ask me about the details, but this offers possibilities if one needs
more power for bleaching, or additional wavelengths next to 470-670nm.
There are some fundamental differences between SP5 and LSM710:
-SP5: white laser, AOBS, STED
-LSM710: option for two scanners, simultaneous spectral detection
Yes, the SP5 detectors are water-cooled. The performance in terms of
signal-to-noise is really nice. But so far I have no comparison to LSM710
(Zeiss claims it's MUCH better than LSM510...).
Personally I found the SP5 to be more user-friendly compared to the LSM510
(straight forward layout and usage); whereas the Zeiss (LSM510) feels more
"scientific" (more values/details given, more complex hardware). Usage on
software-level should be now easier with the Zeiss ZEN software,
unfortunately the first version introduced some instabilities.
Both systems offer fast scanners, which might be interesting for fixed
samples as well (more time-efficient imaging of big/thick samples). Both
systems have single pinholes, so colocalization in the visible range
should not be that tricky. I am still not sure about the laser coupling of
LSM710 - this is now done in the scanhead after the fiber, so how do they
deal with the alignment over multiple wavelengths?
The implementation of the Hamamatsu detector in the old Meta was not
perfect (low sensitivity and signal-to-noise), however, with bright
samples still the second-fastest solution after Nikon. One needs to test
if the new version in the LSM710 does perform better. The beam-path looks
promising.
Michael
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>>
>
>
> Do they offer the option of violet (or other) lasers in addition to
> the supercontinum laser? That would extend it beyond the 470-670 limits?
>
> The brochure also mentions cooled PMTs - I wonder what real-world
> impact that will have?
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
>
>
>>
>> Just a correction .........
>> Leica SP5X tunable white light laser system covers 470-670 nm only,
>> not the entire visible spectrum. Very stable, provide 1.5 mW per nm,
>> need more power... increase the bandwidth.
>> ammasi
>>
>>
>>
>> On Mon, 31 Mar 2008 21:55:26 -0500
>> Mayandi Sivaguru <[log in to unmask]> wrote:
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>> Page, check out also the Leica spectral excitation and spectral
>>> emission confocal SP5 X. This has a single white laser with a
>>> single laser capable of tuning excitation wavelengths at the entire
>>> visible spectrum at 1nm resolution.
>>> In addition, remember the SP5 comes with a resonant scanner too. I
>>> have personally tested a SP5 system with STED with the samples I
>>> have prepared and very pleased with the results. That means if you
>>> have a SP5 with resonant scanner you can latter upgrade to
>>> superresolution technique (STED) which can yield around 90 nm
>>> resolutions in xy. THe AOBS based system is great as you loose less
>>> photons.
>>> But, the Zeiss 710 system's new grating design is worth to have a
>>> look. I personally feel that the Zeiss 710 is a system step cloaser
>>> to a spectral confocal such as an SP5. Zeiss has great flexibility
>>> in your laser selection and that will let you not burn your wallet
>>> upfront and you can add lasers latter as and when it is needed.
>>> No commercial interest on either vendors. I feel both should be
>>> great systems and you should select depending on your necessity and
>>> your budget.
>>> Shiv
>>> At 02:40 PM 3/31/2008, you wrote:
>>>> Search the CONFOCAL archive at
>>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>> Hi Page,
>>>>
>>>> We had a new SP5 installed mid-August 2007 and we have been happy
>>>> with it. The system had the usual teething problems, but Leica
>>>> was rather efficient in fixing them. The software was never
>>>> Leica's strong side, and the new LAS AF is no exception, but you
>>>> learn to live with it; the 1.8.2 version is definitely an
>>>> improvement. The PMTs are great with a wonderful STN ratio, and
>>>> the objectives are very good as well (the system shows practically
>>>> no lateral or axial chromatic aberration, e.g.). I don't know the
>>>> 710.
>>>> Hope this helps!
>>>>
>>>> Zoltan
>>>>
>>>> On Mon, Mar 31, 2008 at 7:28 PM, D. Page Baluch
>>>> <<mailto:[log in to unmask]
>>>> >[log in to unmask]> wrote:
>>>> Search the CONFOCAL archive at
>>>> <http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We are
>>>> looking to upgrade our facility with a new confocal system. For
>>>> the past 10 years we have used both the Leica TCSNT and SP2
>>>> systems and have been quite satisfied with their performance. We
>>>> are now leaning towards the Leica SP5 but we would like to hear
>>>> from others about their experiences with other confocal systems
>>>> and why they think one might be better than another. We recently
>>>> saw a demo of the Zeiss LSM 710 which appears to be a good system
>>>> and know of some researchers who have used the 510 and love it.
>>>> Most of our users have fixed samples and use an upright microscope.
>>>> Thanks for your suggestions and advice,
>>>> Page
>>>>
>>>> --
>>>> Page Baluch, Ph.D.
>>>> W.M. Keck Bioimaging Lab Manager
>>>> Arizona State University/SoLS
>>>> PO Box 874501
>>>> Tempe, AZ 85287-4501
>>>>
>>>>
>>>>
>>>>
>>>> --
>>>> --
>>>> Zoltan Cseresnyes
>>>> Facility manager, Imaging Suite
>>>> Dept. of Zoology University of Cambridge
>>>> Downing Street, Cambridge
>>>> CB2 3EJ UK
>>>>
>>>> Tel.: (++44) (0)1223 769282
>>>> Fax.: (++44) (0)1223 336676
>>> Mayandi Sivaguru, PhD, PhD
>>> Microscopy Facility Manager
>>> 8, Institute for Genomic Biology
>>> University of Illinois at Urbana-Champaign
>>> 1206 West Gregory Dr.
>>> Urbana, IL 61801 USA
>>> Office: 217.333.1214
>>> Fax: 217.244.2496
>>> [log in to unmask]
>>> http://core.igb.uiuc.edu
>>
>> Ammasi Periasamy, Ph.D.
>> Director, Keck Center for Cellular Imaging (KCCI)
>> Professor of Biology and Biomedical Engineering
>> Biology, Gilmer Hall (064), McCormick Rd
>> University of Virginia
>> Charlottesville, VA 22904
>> Voice: 434-243-7602 (Office); 982-4869 (lab)
>> Fax:434-982-5210; Email:[log in to unmask]
>> http//:www.kcci.virginia.edu
>> ************************
>> Workshop on FRET Microscopy, March 3-7, 2009
>> http://www.kcci.virginia.edu/workshop/workshop2009/index.php
>> *************************
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