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Not 0.3 but 1/3 of course


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Judy Trogadis
Sent: Monday, April 07, 2008 11:03 AM
To: [log in to unmask]
Subject: image processing

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I have a simple question, related to an annoying problem.

I have an image of a multilabelled fluorescent specimen, displayed as a
merged RGB image. I would like to analyze just the red channel, so I
split the channels and obtain B & W images of the individual component.
They all look as bright as the original.

However, if I only have a single red image and would like to convert it
to a B & W image (change mode RGB to grey), for 8-bit analysis in
ImageJ, for example, the resulting image is very dull and fainter.
Furthermore, this effect is much more dramatic with a red image than
with a green image.

What is happening? 
Thanks,
Judy


Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph:  416-864-6060  x6337
pager: 416-685-9219
fax: 416-864-6043
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