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Hye all,


There is a lot if good information about that subject in the microscopy site.

http://www.microscopyu.com/articles/digitalimaging/colorbalance.html

John Russ, is also a man with lot of practical experience in this  
field. He wrote a book on Image processing and several good reviews on  
color photography.

Bye,


Patrick Van Oostveldt

Quoting Judy Trogadis <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I have a simple question, related to an annoying problem.
>
> I have an image of a multilabelled fluorescent specimen, displayed   
> as a merged RGB image. I would like to analyze just the red channel,  
>  so I split the channels and obtain B & W images of the individual   
> component. They all look as bright as the original.
>
> However, if I only have a single red image and would like to convert  
>  it to a B & W image (change mode RGB to grey), for 8-bit analysis  
> in  ImageJ, for example, the resulting image is very dull and  
> fainter.  Furthermore, this effect is much more dramatic with a red  
> image than  with a green image.
>
> What is happening?
> Thanks,
> Judy
>
>
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 7Queen
> 30 Bond St.
> Toronto, ON M5B 1W8, Canada
> ph:  416-864-6060  x6337
> pager: 416-685-9219
> fax: 416-864-6043
> [log in to unmask]
>



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