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Hi,

anybody knows if FDA (fluorescein diacetate) 
distributes evenly within cells once incorporated 
and cleaved by cellular esterases?

I assume that once cleaved, FDA can no longer 
cross membranes and therefore remains trapped in soluble compartments.

In other words, can FDA be used as a marker of 
internal cell volume due to its free diffusion 
among continuous cellular compartments (Vg. cytosol + nuclear matrix)?

Thanks


F Javier Diez-Guerra, PhD
Profesor Titular
Centro de Biologia Molecular Severo Ochoa
C/ Nicolás Cabrera, 1
Universidad Autónoma
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN

phone:  +34 91 196 4612
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